General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS HTR1F β-Arrestin Cell Line, 2 vials. (93-0699C3)
The PathHunter HTR1F cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 CALCRL-RAMP3 β-Arrestin Cell Line, 2 vials. (93-0252C2)
The PathHunter CALCRL-RAMP3 (AM2) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Pancreas, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Gallbladder, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Heart: Ventricle (left), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Sigma Aldrich Fine Chemicals Biosciences Human Dermal fibroblasts
Dermal fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin and play a crucial role during wound healing. HDf provide an excellent model system to study many aspects of cell physiology and have been utilized in dozens of research publications particularly those related to skin biology and reprogramming/induced pluripotency studies. HDf were used to create along with human synovyocytes induced pluripotent stem cells (iPSC) by using the now classic Yamanaka cocktail of Oct3/4 Sox2 Klf4 and c-Myc the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi 2007 2009) and to characterize iPSC general properties miRNA expression profiles and differentiation potential (Hayashi 2010 Ohta 2011 Koyanagi-Aoi 2013 Noguchi 20130 Razak 2013 Sakurai 2013 Tanaka 2013 Yanagida 2013)
Biochain Institute Inc Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Lung, 5 x2 slides /PK
BioChain's human paraffin tissue (FFPE) section matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of paraffin tissue sections of primary tumor and its adjacent normal tissue; PM consists of paraffin tissue sections of primary tumor and corresponding metastatic tumor. Paraffin tissue sections in each pair are prepared from the same donor. This product line is ideal for localization of tumor- specific genes and tumor metastatic genes.
ATCC Quantitative; Genomic DNA; from BK Polyomavirus
Quantitative Genomic DNA from BK Polyomavirus can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biochain Institute Inc Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue I, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. The tissues are immediately put in liquid nitrogen after being excised, and then identified by a board-licensed pathologist. Patient demographic and disease related history data are available. BioChain's Frozen Tissue Panels are manufactured using high-quality tissues. 5 to 8 different tissues are mounted on a modified glass slide after preparation with proprietary techniques. Each section is approximately 5 um thick. H.E. staining, immunostaining, and in situ hybridization with proliferating cellular nuclear antigen (PCNA) antibody and P53 gene probe ensure the quality of tissue panels. It is an ideal product for rapid cellular localization of RNA and protein expression.
Biochain Institute Inc Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues, 5 slides/PK
BioChain's neurological diseased frozen tissue panels are designed for studying Alzheimer, Parkinson's Disease, Dementia, Multiple Sclerosis, Progressive Supranuclear Palsy (PSP), and Depression diseases. 6-8 same diseased frozen tissues with 5-10 um thickness are mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Appendix, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Frozen Tissue Section - Arrhythmia, infarct: Heart Ventricle, right, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biolegend Spark Violet™ 423 anti-rat CD3 [1F4]; Isotype: Mouse IgM, κ; Reactivity: Rat; Apps: FC; Size: 100 μg
Spark Violet™ 423 anti-rat CD3 [1F4]; Isotype: Mouse IgM, κ; Reactivity: Rat; Apps: FC; Size: 100 μg
Eurofins Discoverx Ready-to-Assay CaS Calcium Sensor Receptor Frozen Cells, 2 Vials of Cells. (HTS137RTA)
The Ready-to-Assay CaS Calcium Sensor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses CXCR7 based activation of CASR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CASR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CASR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.